A.V. Luikov Institute of Heat and Mass Transfer

http://www.itmo.by/  
220072 Minsk, Brovki str. 15

Research complex for the analysis of biological cells in vitro by optical, fluorescent and atomic force microscopy

COUNTRY OF ORIGIN

IDENTIFIER

TO6651

PUBLISHED

2023-04-23

LAST UPDATE

2023-04-23

DEADLINE

Linked profile in other language
Responsible
Svetlana Markova
+375 29 752 8328
sveta_oms@itmo.by
Summary
A.V.Luikov Heat and Mass Transfer Institute offers consumers a research complex for the analysis of biological cells in vitro by optical, fluorescent and atomic force microscopy under a commercial agreement with technical assistance.
Description
The research complex is intended for registration of optical, fluorescent and AFM images of biological objects at the micro- and nanolevel, for evaluation of local elasticity and adhesion ability of cells. The complex can be used to study micro- and nanoscale objects and analyze the surface of polymeric and composite materials.

The complex includes:
- modular scanning atomic force microscope (SAM, combined with a fluorescent optical microscope);
-specialized software for AFM (SurfaceScan Microtestmachines) and fluorescence microscopy (Microvisible ver. 6.3.1 MICROS);
-system for ensuring the vital activity of cells (temperature control of the sample with the maintenance of a given composition of the gaseous atmosphere).

Basic techniques of AFM and fluorescence microscopy:
-contact static atomic force microscopy;
-lateral force microscopy;
-semi-contact dynamic atomic force microscopy;
-phase contrast microscopy and fluorescence microscopy;
-support for TIRFM-, STED-, RESOLFT-, PALM- fluorescence microscopy techniques.

It is possible to use standard culture dishes - Petri dishes with a diameter of 25 mm (bottom thickness up to 1 mm) and standard glass slides. ACM uses a specialized chip holder with the ability to quick change of pre-installed cantilever chips with a standard size of 1.6x3.4x0.3 mm. It is possible to install a chip holder according to customer requirements (isolated holder, fiber optic illuminator, fiber optic spectrometer, etc.).

Main technical characteristics of the complex:
-optical magnification up to 300X;
-resolution in the AFM mode 2-10 nm;
-scanning area in the AFM mode 100×100 µm;
-temperature in the working area of ​​the sample 24-27°С±0.5°С;
-time of continuous operation with temperature control of the sample up to 170 hours.

Optical magnification is determined by the type and configuration of the fluorescent microscope used. Type, brand and complete set of a fluorescent microscope can be changed according to the customer's request. In the basic configuration, an inverted microscope MICROS Sundew MCXI600 is installed.

The Institute offers its partners, under a commercial agreement with technical support, the acquisition of a research complex for the analysis of biological cells in vitro by optical, fluorescence and atomic force microscopy, the development of technical tools and methods for examining samples at the request of the customer.
Advantages and Innovations
The advantages of the complex are:
-open architecture;
-modular structure;
-use of cooled CCD receivers for registration of fluorescent images;
-use of standard protocols and probes;
- control of CO2 concentration and sample temperature in the working volume.
Stage of development
Field tested/evaluated (TRL8)
Comments regarding stage of development
The research complex has been developed and is used in medical and biological research. Design documentation for the units of the installation.
Funding source
State budged
Internal
IPR status
Exclusive rights
Secret know-how
Sector group
BioChemTech
Healthcare
Nano and micro technologies

Organization information

Type
R&D institution
Year established
1952
NACE keywords
C.28.99 - Manufacture of other special-purpose machinery n.e.c.
M.72.19 - Other research and experimental development on natural sciences and engineering
M.74.90 - Other professional, scientific and technical activities n.e.c.
Turnover (in EUR)
1-10M
Already engaged in transnational cooperation
Yes
Languages spoken
English
Russian

Information about partnership

Type of partnership considered
Commercial agreement with technical assistance
Type and role of partner sought
Consumers interested in purchasing a research complex for the analysis of biological cells in vitro by optical, fluorescence and atomic force microscopy under a commercial agreement with technical assistance.
Type and size of partner sought
> 500
251-500
SME 51-250
SME 11-50
SME <= 10
R&D Institution
University

Attachments

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Statistics since 24.04.2023 01:47:30