Latest AIM Projects


Use of Ophthalmic Acid for treatment of Parkinson's disease

The use of Ophthalmic acid provides a therapeutic target for more efficient and long duration of treatment of Parkinson’s Disease. Animal studies have shown that central administration of OA can reverse PD symptoms for prolonged duration. Further, OA precursor 2 aminobutyrate was able to increase and prolong motor activity when administered peripherally with L-DOPA/NSD1015. OA acts through activating Calcium sensing receptors.


Ultra-low Voltage EDA Acquisition Circuits with an Adaptive Feedback System

Utilizing a novel adaptive gain mechanism and operating on a low voltage, the system captures electrodermal activity (EDA) signals, which are reliable stress indicators. It is designed to overcome the limitations of current wearable devices by offering high sensitivity and low power consumption, making it ideal for continuous stress monitoring.


Velocity-based Clinical Optoretinography System

Over 2.2 billion people suffer from eye disease, leading to near or distant vision impairment. Unfortunately, in at least 1 billion of these cases, vision impairment could have been prevented or has yet to be addressed. In the US, eye disease leads to more than $139 billion in economic burden. The most common eye conditions include age-related macular degeneration, glaucoma, diabetic retinopathy, and cataracts. Optical coherence tomography (OCT) is a noninvasive imaging modality used throughout medicine that generates images of biological tissues with high axial and transverse resolutions. While it is a standard for diagnosing eye conditions, it still suffers from limited resolution, large file size, high technical expertise, and expensive systems.

Researchers at the University of California, Davis, have developed a new optoretinography system using tissue velocity obtained from a modified OCT system. The system avoids the need to track specific cells over time, obviates the cost and labor of the position-based approaches such as adaptive optics, digital aberration correction and real time tracking. The system extracts OCT images within 40 milliseconds and produces optoretinograms, a measurement of neural function in the retina (e.g., photoreceptors). A prototype of the system has been developed, and responses have been acquired from three test subjects. Results indicate the system exhibits high test-retest repeatability and dependence on stimulus dose and retinal eccentricity.


Biomaterial For Wound Healing

This technology presents a novel wound-healing hydrogel comprising a gelatin scaffold encompassing apoptotic neutrophils. Prototype studies, within a wound-healing animal model, have shown that treatment with the GelMA-AN improved wound healing, vasculature length and cell growth compared to control hydrogels. In addition, GelMA-AN may also stimulate production of wound-healing cytokines and other immune mediators. GelMA-AN represents a low-cost biocompatible regenerative treatment for chronic wounds.


Heterologous Synthesis of Nitrogenase in E. coli

Biological nitrogen fixation (BNF) offers a natural means of supplying fixed nitrogen to crops. Catalyzed by a metallo-enzyme called nitrogenase, the BNF process converts N2 to NH3 at ambient conditions, circumventing the problems of fossil-fuel consumption and carbon-waste production associated with its industrial parallel while reducing the nitrogen-cycle perturbance and the surface/ground-water pollution caused by the excess application of commercial ammonia.However, BNF bacteria are difficult to grow and not suitable for all plant conditions.The expression of nitrogenase in an organism that can be cultured and mass-produced in a lab is a long-sought-after, yet non-trivial task given the necessity to introduce a large number (more than 20) of nitrogen fixation (nif) and related genes into a new host.Researchers have completed heterologous synthesis of two simplified nitrogenase analogs from Azotobacter vinelandii in E. coli. One analog consists of the reductase component (NifH) and, in place of the catalytic component (NifDK), a cofactor maturase (NifEN); whereas the other analog consists of only NifEN.Both systems mimic the nitrogenase enzyme in reducing N2 and incorporating the reduced N into the cellular mass.


Scalable and Eco-Friendly Manufacturing of Tunable Heterogeneous Nanoparticle Films

A scalable heterogeneous nanoparticle film with controllable manufacturing methods with tunable properties that could be useful for a variety of applications.
Problem:
Current techniques for manufacturing polymer-infiltrated nanoparticle films (PINFs) include immersion in polymer melts, capillary rise infiltration, and solvent-driven infiltration. These methods only produce homogenous PINFs that have a uniform composition and structure throughout the thickness. Heterostructured PINFs with varying structures would be useful for certain applications including drug delivery, membrane separation, and photonics, but there is a lack of scalable and controllable methods to produce such PINFs.
Solution:
The inventors developed a scalable and controllable method to produce tunable heterostructued PINFs with a high-volume fraction of nanoparticles. The thickness and porosity of these PINFs can be modulated, creating films with varying physical and optical properties that can be customized for different applications. Additionally, the method does not use organic solvents, making it eco-friendly.
Technology:
A layer of silicon dioxide (SiO2) nanoparticles is spin-coated onto a layer of larger polystyrene nanoparticles. These dispersions may also be mixed and blade-coated in one step for better manufacture scaling. The bilayer is heated above polystyrene’s glass temperature (100⁰C), and the polystyrene particles melt and wick into the SiO2 layer. The space occupied by the polystyrene nanoparticles is left void of SiO2 nanoparticles. The film’s refractive index can be tuned by changing the relative volume of inorganic nanoparticles, and therefore the relative pore size. Films of alternating refractive indices can be stacked to form a Bragg reflector.
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The figure shows SEM images before and after nanocomposite annealing. SiO2 nanoparticles are layered on polystyrene particles, which melt and form a matrix upon solvent-driven annealing. This leaves the space previously occupied by SiO2 unoccupied. The films can be produced by using either a blade or a spinner to coat the substrate.

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Docket #20-9443


A Linear Coding Method for Communication in Networks

­Leveraging data from generic sensing devices to enable accurate crowd-sourced road geometry estimation and enhanced vehicular state estimations. Background: In the transportation industry, key challenges revolve around obtaining road geometry data and reliable vehicular state estimation using generic sensing devices.  These devices, while widely used for navigation and other applications, face limitations such as sensor accuracy and variability in device placement within vehicles.  Traditional methods often struggle to compensate for these challenges, leading to inaccuracies in localization and navigation, particularly in urban areas or environments with obstructed GPS signals. Technology Overview: This University at Buffalo technology improves road geometry estimation and vehicular state tracking by harnessing data from common sensing devices like smartphones and tablets.  It employs techniques to fuse data from accelerometers, gyroscopes, and GPS, enabling accurate estimation of road features and vehicle states.  The technology utilizes the gyroscope as the primary sensor for estimating road geometry features likes grade, elevation, and transverse slope.  Secondly, it leverages remote sensing elevation data and road design principles to estimate accelerometer bias, addressing challenges related to arbitrary sensor placement within the vehicle  Thirdly, it implements quality-aware crowd-sourcing and decouples estimations from accelerometer and gyroscope data from different trips, ensuring more accurate road geometry estimations. https://buffalo.technologypublisher.com/files/sites/7520_in-part_image.jpg Source: kosssmosss, https://stock.adobe.com/uk/390186275, stock.adobe.com Advantages: Applications: Applications: Intellectual Property Summary: U.S. patent application 18/633,500 filed April 11, 2024. Stage of Development: Technology Readiness Level (TRL): 6 Licensing Status: Available for licensing or collaboration. Figure 1: https://buffalo.technologypublisher.com/files/sites/7520_image.jpg


Immune Modulator Encoded Lipid Nanoparticle Formulation Improves T Cell Response to Vaccines

Researchers developed an LNP-mRNA-based vaccine that encodes different cytokine mRNAs, as a method to enhance CD8+ T cell responses and memory formation.
Problem:
Lipid nanoparticles (LNPs) are an emerging technique to deliver mRNA in vivo more efficiently. While recent progress in mRNA technology has allowed the rapid development of SARS-COV2 vaccines, these protective responses often require high doses and boosting to generate long lived protective T cell responses.
Solution:
Dr. Hunter and his team have developed a method to promote T-cell immune responses to vaccines, by delivering mRNA that encodes cytokine (IL-12 or IL-27) concurrently with the antigen encoding mRNA. With this approach, they enable the future development of new vaccines by “mixing and matching” target antigens with different cytokines to enhance the memory T cell pool.
Technology:
The inventors previously discovered the importance of IL-27 in eliciting immune responses of CD8+ T cells post-mRNA LNP immunization. To validate this vaccine approach, the inventors first test IL-27 overexpression in vivo with a low dose of the antigen encoding mRNA. They then test the effects of the cytokine/antigen combination vaccine for restoring T cell expansion in IL-27 knock-out mice demonstrating the ability to produce functional cytokine in vivo. In wildtype mice administration of the cytokine/antigen combination vaccine improves the size of the CD8+ T cell memory pool, which is essential for long-term protection. Further, inclusion of cytokine mRNA improved the therapeutic efficacy of an anti-tumor antigen vaccine. Similar studies utilizing IL-12 mRNA showed that IL-12 could also be leveraged to improve memory CD8+ T cell formation and therapeutic efficacy of mRNA-LNPs to malignancy.
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Docket: 22-10096


Walnut Pellicle and Somatic Embryo Tissues as a Unique Plant Source of Bioactive Lipid Supplements

This technology focuses on the enrichment of fatty acid compounds and bioactive lipids from walnut tissues and somatic embryos, targeting unconventional lipids with potential molecular bioactivities beneficial to human and plant health. Leveraging the nutritional and health-promoting aspects of walnuts, the method aims to harness specific fatty acids and lipids for their roles in reducing cardiovascular diseases, cancer risk, metabolic dysfunctions, and supporting cognitive as well as gut health through epigenetic cellular mechanism.


Meristem Transformation Method Using A Liquid Selection Medium

UW-Madison researchers have developed a method for genetically manipulating cannabis that is less laborious and more efficient than previous methods. The new method involves retaining both primary leaves in the cannabis explant, culturing the explant in a liquid media rather than on a solid media, and using spectinomycin at the rooting stage along with a specialized rooting media. The researchers used agrobacterium to transfect the plant with a gene encoding a red fluorescent protein. This method cut the time between excising the explant to the greenhouse in half as compared to their initial method and improved transformation frequency 5-10x higher than the previous method the researchers used.